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Jensen AcklesBruce Wayne / Batman (voice)
Josh DuhamelHarvey Dent / Two-Face (voice)
Naya RiveraSelina Kyle / Catwoman (voice)
Billy BurkeCommissioner James Gordon (voice)
Titus WelliverCarmine 'The Roman' Falcone / Luigi Maroni (voice)
David DastmalchianJulian Day / Calendar Man / Oswald Cobblepot / Penguin (voice)
Julie NathansonGilda Dent (voice)
Alastair DuncanAlfred Pennyworth (voice)
Katee SackhoffPamela Isley / Poison Ivy (voice)
Robin Atkin DownesJonathan Crane / Scarecrow / Thomas Wayne (voice)
John DiMaggioJervis Tetch / Mad Hatter (voice)
Laila BerzinsSofia Falcone (voice)
Fred TatascioreSolomon Grundy / Vincent Falcone (voice)
Amy LandeckerBarbara Gordon / Carla Viti (voice)
Troy BakerJoker (voice)
Alyssa DiazRenee Montoya (voice)
Gary LeRoi GrayPearce (voice)
Jim PirriSal Maroni (voice)
Zach CallisonYoung Bruce Wayne (voice)
Rick D. WassermanBodyguard (voice)
